Bio Elpida, Isolation of Primary Cells
Cloning, differentiation and selection of the best candidate cell lines
Bio Elpida can perform isolation of primary cells from human samples
. The company has developed efficient processes for the cloning, differentiation and selection of the best candidate cell lines
Our team of experts can also develop:
- Material for tissues cell bank
- Cell banks: research cell bank Master Cell Bank, Working Cell Bank…
- Regenerative therapies
All steps can be performed in our pharmaceutical zone and can lead to the delivery of a good cells culture pratices certificate.
The techniques used are:
- Mechanical isolation, without trypsination, for tissues, organs or biological samples (biopsy, tumour…)
- Classical techniques for selection of cells from total blood or liquid samples using centrifugation, concentration gradient, coupling to antibodies/magnetic beads…
Cloning is performed by limit dilution.
The cloning step can be performed in the pharmaceutical zone, according to GMP guidelines for the production of pharmaceutical products. This enables Bio Elpida to produces clones that can benefit from a GMP release certificate by our qualified person.